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Insights into the changes of amino acids, microbial community, and enzymatic activities related with

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1635-y

摘要：

● The highest seed germination index was achieved at 0.3 g/g total solids of food waste.

关键词： Composting     Food waste     Amino acids     Microbial community     Enzymatic activity     Nutrient quality    

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Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

《能源前沿（英文）》 2018年 第12卷 第2期   页码 233-238 doi: 10.1007/s11708-018-0529-3

摘要： Heteroatom-doping of pristine graphene is an effective route for tailoring new characteristics in terms of catalytic performance which opens up potentials for new applications in energy conversion and storage devices. Nitrogen-doped graphene (N-graphene), for instance, has shown excellent performance in many electrochemical systems involving oxygen reduction reaction (ORR), and more recently glucose oxidation. Owing to the excellent sensitivity of N-graphene, the development of highly sensitive and fast-response enzymatic biosensors is made possible. However, a question that needs to be addressed is whether or not improving the anodic response to glucose detection leads to a higher overall performance of enzymatic biofuel cell (eBFC). Thus, here we first synthesized N-graphene via a catalyst-free single-step thermal process, and made use of it as the biocatalyst support in a membraneless eBFC to identify its role in altering the performance characteristics. Our findings demonstrate that the electron accepting nitrogen sites in the graphene structure enhances the electron transfer efficiency between the mediator (redox polymer), redox active site of the enzymes, and electrode surface. Moreover, the best performance in terms of power output and current density of eBFCs was observed when the bioanode was modified with highly doped N-graphene.

关键词： enzymatic fuel cell     nitrogen-doped graphene     reduced graphene oxide     catalyst-free synthesis    

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Enzymatic nitrous oxide emissions from wastewatertreatment

Gang Guo, Yayi Wang, Tianwei Hao, Di Wu, Guang-Hao Chen

《环境科学与工程前沿（英文）》 2018年 第12卷 第1期 doi: 10.1007/s11783-018-1021-3

摘要： Nitrous oxide (N O), a potent greenhousegas, is emitted during nitrogen removal in wastewater treatment, significantlycontributing to greenhouse effect. Nitrogen removal generally involvesnitrification and denitrification catalyzed by specific enzymes. N O production and consumption vary considerably in responseto specific enzyme-catalyzed nitrogen imbalances, but the mechanismsare not yet completely understood. Studying the regulation of relatedenzymes’ activity is essential to minimize N O emissions during wastewater treatment. This paper aims to reviewthe poorly understood related enzymes that most commonly involvedin producing and consuming N O in terms oftheir nature, structure and catalytic mechanisms. The pathways ofN O emission during wastewater treatment arebriefly introduced. The key environmental factors influencing N O emission through regulatory enzymes are summarizedand the enzyme-based mechanisms are revealed. Several enzyme-basedtechniques for mitigating N O emissions directlyor indirectly are proposed. Finally, areas for further research onN O release during wastewater treatment arediscussed.

关键词： Nitrous oxide     Mitigation     Enzyme catalysis     Nitrogen removal     Wastewater treatment    

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USING AN AQUEOUS ENZYMATIC METHOD

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 623-634 doi: 10.15302/J-FASE -2021400

摘要：

Schizochytrium sp., a marine microalga, is a potential source of edible oil due to its short growth cycle and rapid lipid accumulation, especially of docosahexaenoic acid. An approach to isolate edible microalgal oil from Schizochytrium sp. using aqueous enzymatic extraction (AEE) was developed. Parameters were optimized by single-factor experiments followed by Box-Behnken design. Proteases were effective in extracting oil. The maximum free oil recovery (49.7%±0.58%) and total oil recovery (68.1%±0.94%) were obtained under optimum conditions of liquid-to-solid ratio of 4.8:1, a 2.5% enzyme concentration of papain and an extraction time of 2.2 h. There was a significant difference (P<0.05) in polyunsaturated fatty acid composition between microalgal oil obtained by AEE and by Soxhlet extraction, with the former having superior physiochemical properties and higher concentrations of bioactive components including total phenolic compounds and total tocopherols. These findings indicate a potential application of AEE for extraction of oil from Schizochytrium sp.

 

关键词： antioxidant activity / aqueous enzymatic extraction / edible microalgal oil / fatty acid composition / physicochemical properties    

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Dissolution and enzymatic hydrolysis of casein micelles studied by dynamic light scattering

LIU Rui, QI Wei, SU Rongxin, ZHANG Yubin, JIN Fengmin, HE Zhimin

《化学科学与工程前沿（英文）》 2007年 第1卷 第2期   页码 123-127 doi: 10.1007/s11705-007-0023-7

摘要： The effects of temperature, ionic strength, and enzymatic hydrolysis on the average hydrodynamic radius () of casein micelles in phosphate buffer were studied by using dynamic light scattering. The results showed that the average value of casein micelles decreased irreversibly during the heating, decreased with the increase of ionic strength in lower ionic strength solution (less than 0.05 mol/L), but opposite in higher ionic strength solution (above 0.1 mol/L). The value of casein increased rapidly during the process of enzymatic hydrolysis, and the structural model of casein micelles in the enzymatic hydrolysis process was also proposed, i.e. the casein micelle changed from compact sphere into unfolded and regularly flocky peptides.

关键词： compact     strength     temperature     regularly flocky     heating    

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Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer cell line

Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA

《化学科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 482-489 doi: 10.1007/s11705-013-1342-5

摘要： In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps: lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions: 50°C, pH 4.0, 2% enzyme/substrate (E/S) ratio, 15 h, and a solid to liquid ratio of 1 ∶ 10. The pectic extract showed a degree of polymerization (DP) profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5–25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen (4-OHT), a classical anticancer drug, which killed 56.5% of the cells.

关键词： pectic extracts     antiproliferative activity     breast cancer     enzymatic treatment    

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Study on enzymatic hydrolysis of steam exploded straw by using shaking ball

SUN Zhanwei, FU Xiaoguo, CHEN Hongzhang, WANG Yanhui, MA Runyu

《化学科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 22-27 doi: 10.1007/s11705-008-0002-7

摘要： A novel method of enzymatic hydrolysis was developed in this paper to produce a high conversion yield and hydrolysis rate. A comparison was described by using three methods of enzymatic hydrolysis and adsorption of steam exploded straw (SEWS): shaking ball in the regime, shaking with stirrer bed, and static state. The most adequate filter-paper activity, speed and reaction time were 3.6 × 10 mol/(smL), 150 r/min and 24 h, respectively, with the reducing sugar yield of 0.43. The results showed that the method of shaking ball produced the highest adsorption, conversion yields and hydrolysis rate of the enzyme. This might be due to the continuous frequency increase of enzyme adsorption and desorption on the substrate surface as well as the relieved end-product inhibition. The morphological variation of SEWS was characterized by environmental scanning electron microscopy (ESEM).

关键词： adequate filter-paper     comparison     conversion     continuous frequency     substrate    

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Valorisation of protein waste: An enzymatic approach to make commodity chemicals? ?

Madura B. A. Kumar, Yuan Gao, Wei Shen, Lizhong He

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 295-307 doi: 10.1007/s11705-015-1532-4

摘要： Protein-rich waste is an abundantly available resource that is currently used mainly as animal feed and fertilizers. Valorisation of protein waste to higher value products, particularly commodity chemicals such as precursors for polymers, has attracted significant research efforts. Enzyme-based approaches, being environmentally-friendly compared to their chemical counterparts, promise sustainable processes for conversion of protein waste to valuable chemicals. This review provides a general overview on valorisation of protein waste and then further summarises the use of enzymes in different stages of the valorisation process—protein extraction and hydrolysis, separation of individual amino acids and their ultimate conversion into chemicals. Case studies of enzymatic conversion are presented for different amino acids including glutamic acid, lysine, phenylalanine, tyrosine, arginine and aspartic acid. The review compares the different enzyme reactors and operation modes for amino acid conversion. The emerging opportunities and challenges in the field are discussed: engineering powerful enzymes and integrating innovative processes for industrial application at a low cost.

关键词： amino acids     protein waste     reactor     conversion     commodity chemicals     enzymes    

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电渗析组合反渗透过程处理头孢氨苄酶法母液废水的研究

阮慧敏,黄杰,李健,沈江南,王家德,高从堦

《中国工程科学》 2014年 第16卷 第7期   页码 42-46

摘要：

利用电渗析技术脱除头孢氨苄酶法母液废水中的盐分，研究了操作电压、浓缩室与淡化室流速比、温度等因素对电渗析脱盐过程的影响，并研究了反渗透膜法处理脱盐母液废水的膜通量变化趋势。实验结果表明，当淡化室、浓缩室流量均为500 L/h（流速比1∶1），操作电压为25 V，温度＜30 ℃时，电渗析在高效脱盐的同时具有理想的有机物截留率达99.50 %以上，能耗3.65 kW · h/kg；反渗透膜法在处理脱盐母液废水时，膜通量衰减较慢，水回收率达到75.67 %。实验结果表明，利用电渗析、反渗透组合工艺处理头孢氨苄酶法母液废水具有良好的可行性。

关键词： 头孢氨苄     废水     电渗析     反渗透    

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Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,

Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar

《环境科学与工程前沿（英文）》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1292-3

摘要： Abstract • A. sydowii strain bpo1 exhibited 99.8% anthracene degradation efficiency. • Four unique metabolic products were obtained after anthracene degradation. • Ligninolytic enzymes induction played vital roles in the removal of anthracene. • Laccase played a crucial role in comparison with other enzymes induced. The present study investigated the efficiency of Aspergillus sydowii strain bpo1 (GenBank Accession Number: MK373021) in the removal of anthracene (100 mg/L). Optimal degradation efficiency (98.7%) was observed at neutral pH, temperature (30℃), biomass weight (2 g) and salinity (0.2% w/v) within 72 h. The enzyme analyses revealed 131%, 107%, and 89% induction in laccase, lignin peroxidase, and manganese peroxidase respectively during anthracene degradation. Furthermore, the degradation efficiency (99.8%) and enzyme induction were significantly enhanced with the addition of 100 mg/L of citric acid and glucose to the culture. At varying anthracene concentrations (100–500 mg/L), the degradation rate constants (k1) peaked with increasing concentration of anthracene while the half-life (t1/2) decreases with increase in anthracene concentration. Goodness of fit (R2 = 0.976 and 0.982) was observed when the experimental data were subjected to Langmuir and Temkin models respectively which affirmed the monolayer and heterogeneous nature exhibited by A. sydwoii cells during degradation. Four distinct metabolites; anthracene-1,8,9 (2H,8aH,9aH)-trione, 2,4a-dihydronaphthalene-1,5-dione, 1,3,3a,7a-tetrahydro-2-benzofuran-4,7-dione and 2-hydroxybenzoic acid was obtained through Gas Chromatography-Mass spectrometry (GC-MS). A. sydowii exhibited promising potentials in the removal of PAHs.

关键词： Aspergillus sydowii     Anthracene     Biodegradation     Polycyclic aromatic hydrocarbons    

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生物表面活性剂的生产与应用

韩双艳,任昌琼,林影

《中国工程科学》 2009年 第11卷 第4期   页码 26-30

摘要：

生物表面活性剂主要由微生物发酵和酶催化下合成，具有表面活性剂基本结构与性质的物质，其除了具有化学表面活性剂的特点外，还具有生物兼容性高、安全无毒性、可被生物降解、不会对环境造成不利影响等优点。随着环保意识的增强和食品安全级别的提高，生物表面活性剂开始应用于食品、医药、化妆品、洗涤剂和石油化工等领域，研究开发各种高效、低成本的生物表面活性剂生产技术成为生物化工的研究热点。

关键词： 生物表面活性剂     微生物选育     酶催化     生物兼容性     生物降解    

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Electrochemical sensor investigation of carbon-supported PdCoAg multimetal catalysts using sugar-containing beverages

Firat Salman, Hilal C. Kazici, Hilal Kivrak

《化学科学与工程前沿（英文）》 2020年 第14卷 第4期   页码 629-638 doi: 10.1007/s11705-019-1840-1

摘要： Novel PdCoAg/C nanostructures were successfully synthesized by the polyol method in order to develop electrocatalysts, related to the glucose sensor performance of the high glycemic index in beverages. The characterization of this novel PdCoAg/C electrocatalyst was performed by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and high-resolution transmission electron microscopy equipped with energy dispersive X-ray. The characterization results revealed that electronic state of the PdCoAg/C electrocatalyst was modified by the addition of the third metal. The electrochemical performances of the sensor were investigated by cyclic voltammetry and differential pulse voltammetry. The prepared enzyme-free sensor exhibited excellent catalytic activity against glucose with a wide detection range (0.005 to 0.35 mmol∙L ), low limit of detection (0.003 mmol∙L ), high sensitivity (4156.34 µA∙mmol ∙L∙cm ), and long-term stability (10 days) because of the synergistic effect between the ternary metals. The glucose contents of several energy drinks, fruit juices, and carbonated beverages were analyzed using the novel PdCoAg/NGCE/C sensor system. These results indicate the feasibility for applications in the foods industry.

关键词： non-enzymatic     glucose detection     ternary metals     glycemic index     beverages    

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Occurrence and migration of microplastics and plasticizers in different wastewater and sludge treatmentunits in municipal wastewater treatment plant

《环境科学与工程前沿（英文）》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1577-9

摘要：

● Reduce the quantifying MPs time by using Nile red staining.

关键词： Microplastics     Municipal wastewater treatment plant     Phthalate esters     Thermal hydrolysis    

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Seasonal and treatment-process variations in invertebrates in drinking water treatment plants

Zhiling Wu, Xianchun Tang, Hongbin Chen

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1354-6

摘要： Abstract • Seasonal and treatment-process variations in invertebrates in a DWTP were analyzed. • The propagation and leakage of invertebrates in BAC filter were the most serious. • Invertebrates can survive and reproduce in chlorine disinfected clear water tanks. • Proportions of endogenous invertebrates increased along the treatment process. Problems associated with excessive propagation and leakage of invertebrates in drinking water have received increasing attention in recent years. We performed a monthly survey of invertebrate abundance and taxa in the effluent of each treatment stage in a drinking water treatment plant between May 2015 and April 2016 and analyzed seasonal and treatment-process variations in invertebrates. The results showed that invertebrate abundances in raw water, effluent of the biological activated carbon (BAC) filter, and finished water significantly correlated with water temperature, whereas no correlation was observed between water temperature and invertebrate abundance in the effluents of the sedimentation tank and sand filter. The dominant taxa in the effluent of each treatment stage were rotifers, nematodes, and crustaceans. The sedimentation tank could efficiently remove invertebrates with an annual average removal rate of 92%. The propagation and leakage of invertebrates occurred in the sand and BAC filters but more seriously in the latter. The average reproduction rate in the BAC filter was 268.8% with rotifers as the taxon that leaked the most. Invertebrate survival and reproduction were also observed in the chlorine-disinfected clean water reservoir with an average reproduction rate of 41.9%. Owing to differences in chlorine resistance, the reproduction ability of the dominant taxa was in the order nematodes>crustaceans>rotifers. The proportion of endogenous invertebrates gradually increased along the treatment process. The average proportion of endogenous invertebrates in the finished water was higher than 79.0%. Our findings suggested that waterworks should pay more attention to endogenous invertebrate growth.

关键词： Invertebrates     Drinking water     Seasonal variations     Treatment process    

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标题 作者 时间 类型 操作

Insights into the changes of amino acids, microbial community, and enzymatic activities related with

期刊论文

Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

期刊论文

Enzymatic nitrous oxide emissions from wastewatertreatment

Gang Guo, Yayi Wang, Tianwei Hao, Di Wu, Guang-Hao Chen

期刊论文

USING AN AQUEOUS ENZYMATIC METHOD

期刊论文

Dissolution and enzymatic hydrolysis of casein micelles studied by dynamic light scattering

LIU Rui, QI Wei, SU Rongxin, ZHANG Yubin, JIN Fengmin, HE Zhimin

期刊论文

Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer cell line

Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA

期刊论文

Study on enzymatic hydrolysis of steam exploded straw by using shaking ball

SUN Zhanwei, FU Xiaoguo, CHEN Hongzhang, WANG Yanhui, MA Runyu

期刊论文

Valorisation of protein waste: An enzymatic approach to make commodity chemicals? ?

Madura B. A. Kumar, Yuan Gao, Wei Shen, Lizhong He

期刊论文

电渗析组合反渗透过程处理头孢氨苄酶法母液废水的研究

阮慧敏,黄杰,李健,沈江南,王家德,高从堦

期刊论文

Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,

Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar

期刊论文

生物表面活性剂的生产与应用

韩双艳,任昌琼,林影

期刊论文

Electrochemical sensor investigation of carbon-supported PdCoAg multimetal catalysts using sugar-containing beverages

Firat Salman, Hilal C. Kazici, Hilal Kivrak

期刊论文

Occurrence and migration of microplastics and plasticizers in different wastewater and sludge treatmentunits in municipal wastewater treatment plant

期刊论文

罗军：Treatment Selection in Prostate Cancer（2019年8月16日）

2021年04月22日

会议视频

Seasonal and treatment-process variations in invertebrates in drinking water treatment plants

Zhiling Wu, Xianchun Tang, Hongbin Chen

期刊论文